Lab and Equipment

Crossing mushroom strains for better yields. Monokaryotic isolation, clamp connection verification, and what hybrid vigor actually means for growers.

An ISO 7 mushroom lab inside a shipping container for under $8,000. HVAC overpressure, spray foam insulation, and ACH calculations included.

Use a Raspberry Pi 5 and TensorFlow Lite to detect contamination before your eyes can. Camera setup, model training, and alert automation.

The right way to take spore prints, make syringes, and store genetics for years. Covers foil vs. glass, hydration, and long-term viability.

How to hold 75-80F for weeks without cooking your grain. DIY incubation chambers, PID controllers, and the metabolic heat fungi generate themselves.

Build your own laminar flow hood for under $300. HEPA H14 filter selection, blower CFM calculations, and the plenum design that actually works.

How to make, store, and expand mushroom liquid cultures. Covers peptone ratios, sugar clarity, and why a $22 stir plate beats a $60 one.

Do you actually need a flow hood, or is a Still Air Box enough? A cost-per-plate comparison with contamination rate data from both setups.

How to isolate fast-growing mushroom genetics on agar through sectoring. Includes transfer protocols and signs of mycelial senescence.